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SOLVED: I would like to understand how to solve this example step-by-step to prepare for an exam: 4.B. Example of Transformation Efficiency Calculation After 100 μl of competent cells are transformed with
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SOLVED: Your bacterial transformation worked and you see 20 colonies! Using the transformation efficiency equation below, calculate the transformation efficiency if 15 μl of plasmid DNA was used. The plasmid DNA concentration
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SOLVED: Calculate the transformation efficiency for the experimental results in the table below. For your calculations, assume that 10 μL of plasmid was used in the experiment and the plasmid concentration was
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SOLVED: What was the transformation efficiency of the competent bacteria that you used in units of (colonies/μg of DNA)? Competent Colonies: 120 Vol. comp. cells plated: 1/10th vol (45 μl) Given: 1
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SOLVED: Transformation efficiency is the quantitative determination of the number of cells transformed per pg of plasmid DNA. In essence, it is an indicator of the success of the transformation experiment. You